Rat RvD1 (Resolvin D1) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat RvD1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RvD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RvD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  RvD1

• Reactivity:

  Rat

• Field of Research:

  Immunology & Inflammation

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat RvD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RvD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RvD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2.5h

• Standard:

  500 pg/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  7.82-500 pg/mL

• Sensitivity:

  3.49 pg/mL