Mouse Phospho AKT S473 (p-AKT) ELISA Kit

Mouse Phospho AKT S473 (p-AKT) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: p-AKT
• Field of Research: Enzyme & Kinase.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10000 pg/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 156.25-10000 pg/mL
• Sensitivity: 49.6 pg/mL