Hamster TNF-a (Tumor Necrosis Factor-alpha) ELISA Kit

Hamster TNF-a (Tumor Necrosis Factor-alpha) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
• Field of Research: Cytokine; Tumor immunity; Infection immunity.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 200 pg/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 3.13-200 pg/mL
• Sensitivity: 1.09 pg/mL