Mouse SREBP1 (Sterol regulatory element-binding protein 1) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SREBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SREBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SREBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SREBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  SREBP1c; SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1

• CAS Number:

  7732-18-5

• Gene Aliases:

  SREBP1c; SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1

• UniProt:

  Q9WTN3

• Field of Research:

  Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SREBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SREBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SREBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SREBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  50 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.79-50 ng/mL

• Sensitivity:

  0.3 ng/mL

• Species:

  Mouse