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Mouse OPA1 (Optic Atrophy 1) ELISA Kit

CAT: 0855-ELK0973-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0973-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OPA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OPA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
MGM1; NPG; NTG; largeG; Dynamin-Like 120 kDa Protein, Mitochondrial; Optic atrophy protein 1
UniProt
P58281
Reactivity
Mouse
Field of Research
Apoptosis
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OPA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OPA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.059 ng/mL

UniProtKB · P58281

Dynamin-like GTPase OPA1, mitochondrial

OPA1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P58281
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Opa1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.6.5.5
Processing
Precursor
Secondary accessions
A6H6Q3, H7BX01, Q3ULA5, Q8BKU7, Q8BLL3, Q8BM08, Q8R3J7
Protein keywords

PTM

AcetylationDisulfide bond

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Domain

Coiled coilTransit peptideTransmembraneTransmembrane helix

Ligand

GTP-bindingLipid-bindingMagnesiumMetal-bindingNucleotide-binding

Molecular function

Hydrolase

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Technical term

Reference proteome

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