Sheep ADIPOQ (Adiponectin) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep ADIPOQ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep ADIPOQ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep ADIPOQ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep ADIPOQ in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  ADP; GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

• Gene Aliases:

  ADP; GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

• Reactivity:

  Sheep

• Field of Research:

  Metabolic pathway, Infection immunity, Cardiovascular biology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep ADIPOQ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep ADIPOQ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep ADIPOQ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep ADIPOQ in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  200 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids.

• Detection Range:

  3.13 - 200 ng/mL

• Sensitivity:

  1.2 ng/mL

• Species:

  Sheep

• CAS Number:

  7732-18-5