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Mouse IL4 (Interleukin 4) ELISA Kit

CAT: 0855-ELK1153-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1153-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IL-4; BSF1; BCGF1 ; B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
UniProt
P07750
Reactivity
Mouse
Field of Research
Cytokine; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
3.3 pg/mL

UniProtKB · P07750

Interleukin-4

IL4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P07750
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-4
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

AutophagyB-cell activation

Molecular function

CytokineGrowth factor

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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