Products for Research Use Only
RegisterLogin

Human apoTf (Apotransferrin) ELISA Kit

CAT: 0855-ELK1285-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK1285-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human apoTf. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human apoTf. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human apoTf, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human apoTf in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Apo-Transferrin; Apo-Tf
UniProt
P02787
Reactivity
Human
Field of Research
Metabolic pathway; Tumor immunity; Hepatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human apoTf. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human apoTf. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human apoTf, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human apoTf in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.071 ng/mL

UniProtKB · P02787

Serotransferrin

TRFE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02787
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Transferrin
EC number
—
Processing
Precursor
Secondary accessions
O43890, Q1HBA5, Q9NQB8, Q9UHV0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Disease variant

PTM

Disulfide bondGlycoproteinMethylationPhosphoprotein

Biological process

Ion transportIron transportTransport

Ligand

IronMetal-binding

Domain

RepeatSignal

Cellular component

Secreted

Related Products

CatalogName
ELK2250-01Human APOF (Apolipoprotein F) ELISA Kit
ELK2250MS-01Human APOF (Apolipoprotein F) Microsample ELISA Kit
ELK6410-01Rat APOF (Apolipoprotein F) ELISA Kit
ELK8409-01Human Catestatin ELISA Kit
ELK10769-01Human Irisin ELISA Kit
ELK1682-01Human ON (Osteonectin) ELISA Kit
ELK9826-01Human Adalimumab ELISA Kit
ELK8382-01Human Barbiturates ELISA Kit
ELK8383-01Human Bisphenol A ELISA Kit
ELK8393-01Human STOML2 ELISA Kit

Alternative Products

CatalogName
EA0131HuHuman apotransferrin, apoTf ELISA KIT
MBS8800287-01Human apoTf (Apotransferrin) ELISA Kit
BG-HUM10290Human Apotransferrin ApoTf ELISA Kit
GA-E1160HM-01Human apotransferrin (apoTf) ELISA Kit
TRV-046251-01ELISA Kit for Apotransferrin (apoTf)
RK00929Human Apotransferrin ELISA Kit (apoTf)
JOT-EK7172Hu-01Human Apotransferrin,APOTF ELISA KIT
KBH20172GENLISA™ Human Apotransferrin (apoTf) ELISA
B6331Apotransferrin (apoTf) Human ELISA Kit
BG-MUS10305Mouse Apotransferrin ApoTf ELISA Kit

Popular Products