Mouse ITaC (Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITaC in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine (C-X-C-Motif) ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9

• Gene Aliases:

  CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine(C-X-C-Motif)ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9

• UniProt:

  Q9JHH5

• Reactivity:

  Mouse

• Field of Research:

  Cytokine, Signal transduction, Infection immunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITaC in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  2000 pg/mL

• Sample Type:

  Serum, plasma and other biological fluids.

• Detection Range:

  31.25 - 2000 pg/mL

• Sensitivity:

  12.7 pg/mL

• Species:

  Mouse

• CAS Number:

  7732-18-5