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Rat MBP (Myelin Basic Protein) ELISA Kit

CAT: 0855-ELK1485-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1485-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MBP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Myelin A1 protein; Myelin membrane encephalitogenic protein
UniProt
P02688
Reactivity
Rat
Field of Research
Infection immunity; Neuro science; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MBP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · P02688

Myelin basic protein

MBP_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P02688
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mbp
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
MBP
EC number
—
Processing
—
Secondary accessions
Q505J1, Q8R4K6, Q9Z1J4, Q9Z1J5, Q9Z1J6
Protein keywords

PTM

AcetylationCitrullinationMethylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Autoimmune encephalomyelitis

Cellular component

Cell membraneMembrane

Technical term

Direct protein sequencingReference proteome

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