Human aLA (Alpha-Lactalbumin) ELISA Kit

Human aLA (Alpha-Lactalbumin) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aLA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aLA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human aLA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aLA in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: LALBA; LYZL7; Lactose Synthase B Protein; Lysozyme Like Protein 7
• UniProt: P00709
• Field of Research: Tumor immunity; Infection immunity; Endocrinology; Reproductive science; Hormone metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 100 ng/mL
• Sample Type: Breast milk and other biological fluids
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.67 ng/mL