Rat KLK4 (Kallikrein 4) ELISA Kit

Rat KLK4 (Kallikrein 4) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KLK4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLK4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KLK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLK4 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: ARM1; EMSP1; KLK-L1; PRSS17; PSTS; Enamel Matrix,Prostate; Kallikrein-Related Peptidase 4; Enamel matrix serine proteinase 1; Serine protease 17; Kallikrein-like protein 1
• UniProt: Q6IE12
• Field of Research: Enzyme & Kinase; Tumor immunity.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Serum, plasma and other biological fluids.
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.05 ng/mL