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Mouse C1INH (Complement 1 Inhibitor) ELISA Kit

CAT: 0855-ELK2339-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2339-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C1INH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C1INH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C1INH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C1INH in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SERPING1; C1IN; C1-INH; C1NH; HAE1; HAE2; Serpin Peptidase Inhibitor Clade G Member 1; Angioedema Hereditary; C1 Inhibitor; C1 Esterase Inhibitor
UniProt
P97290
Reactivity
Mouse
Field of Research
Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C1INH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C1INH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C1INH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C1INH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 ng/mL
Sensitivity
6 ng/mL

UniProtKB · P97290

Plasma protease C1 inhibitor

IC1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P97290
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Serping1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
C1 Inh, C1Inh
EC number
—
Processing
Precursor
Secondary accessions
A2ATR7, O88330, Q99M43, Q9QX09
Protein keywords

Biological process

Blood coagulationFibrinolysisHemostasis

PTM

Disulfide bondGlycoprotein

Molecular function

Protease inhibitorSerine protease inhibitor

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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