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Human TG (Thyroglobulin) ELISA Kit

CAT: 0855-ELK2353-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2353-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TG in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
AITD3; TGN
UniProt
P01266
Reactivity
Human
Field of Research
Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TG in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.138 ng/mL

UniProtKB · P01266

Thyroglobulin

THYG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01266
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TG
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Tg
EC number
—
Processing
Precursor
Secondary accessions
O15274, O43899, Q15593, Q15948, Q9NYR1, Q9NYR2, Q9UMZ0, Q9UNY3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

Congenital hypothyroidismDisease variant

PTM

Disulfide bondGlycoproteinIodinationProteoglycanSulfation

Molecular function

HormoneThyroid hormone

Domain

RepeatSignal

Cellular component

Secreted

Biological process

Thyroid hormones biosynthesis

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