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Mouse ICAM2 (Intercellular Adhesion Molecule 2) ELISA Kit

CAT: 0855-ELK2586-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2586-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ICAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ICAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ICAM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD102
UniProt
P35330
Reactivity
Mouse
Field of Research
CD & Adhesion molecule; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ICAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ICAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ICAM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.073 ng/mL

UniProtKB · P35330

Intercellular adhesion molecule 2

ICAM2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P35330
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Icam2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
ICAM-2
EC number
—
Processing
Precursor
Secondary accessions
Q9D8Q4
Protein keywords

Technical term

3D-structureReference proteome

Biological process

Cell adhesion

Cellular component

Cell membraneCell projectionMembrane

PTM

Disulfide bondGlycoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix