Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2
• UniProt: P35355
• Field of Research: Enzyme & Kinase; Tumor immunity; Infection immunity; Reproductive science; Pulmonology; Gastroenterology; Hormone metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.065 ng/mL