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Mouse PGC (Pepsinogen C) ELISA Kit

CAT: 0855-ELK2861-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2861-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PGC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PG-C; Progastricsin; Gastricsin; Pepsinogen II; Pepsinogen C
UniProt
Q9D7R7
Reactivity
Mouse
Field of Research
Enzyme & Kinase; Tumor immunity; Gastroenterology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PGC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.064 ng/mL

UniProtKB · Q9D7R7

Gastricsin

PEPC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9D7R7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pgc
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.23.3
Processing
Precursor
Secondary accessions
Q9D7T2
Protein keywords

Molecular function

Aspartyl proteaseHydrolaseProtease

Biological process

Digestion

PTM

Disulfide bondZymogen

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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