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Human DCX (Doublecortin) ELISA Kit

CAT: 0855-ELK2898-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2898-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DCX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DCX in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
DBCN; DC; LISX; SCLH; XLIS; Doublin; Doublecortex; Lissencephalin-X; Lissencephaly, X-Linked; Neuronal migration protein doublecortin
UniProt
O43602
Reactivity
Human
Field of Research
Endocrinology; Neuro science; Hormone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DCX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DCX in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.058 ng/mL

UniProtKB · O43602

Neuronal migration protein doublecortin

DCX_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43602
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DCX
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A6NFY6, A9Z1V8, D3DUY8, D3DUY9, D3DUZ0, O43911, Q5JYZ5
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Cellular component

Cell projectionCytoplasmMicrotubule

Molecular function

Developmental protein

Biological process

DifferentiationNeurogenesis

Disease

Disease variantEpilepsyLissencephaly

PTM

PhosphoproteinUbl conjugation

Domain

Repeat

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