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Human MMRN1 (Multimerin 1) ELISA Kit

CAT: 0855-ELK3228-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3228-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMRN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMRN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMRN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMRN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
ECM; EMILIN4; GPIa; MMRN; Glycoprotein Ia; Elastin microfibril interface located protein 4; Endothelial cell multimerin; Platelet glycoprotein Ia; 155 kDa platelet multimerin
UniProt
Q13201
Reactivity
Human
Field of Research
Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMRN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMRN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMRN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMRN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.62 ng/mL

UniProtKB · Q13201

Multimerin-1

MMRN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13201
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MMRN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q4W5L1, Q6P3T8, Q6ZUL9
Protein keywords

Coding sequence diversity

Alternative splicing

Domain

Coiled coilEGF-like domainSignal

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

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