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Human COMT (Catechol-O-Methyltransferase) ELISA Kit

CAT: 0855-ELK3245-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3245-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COMT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COMT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Catechol O methyltransferase; Catechol O-methyltransferase; COMT
UniProt
P21964
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COMT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COMT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.064 ng/mL

UniProtKB · P21964

Catechol O-methyltransferase

COMT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P21964
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
COMT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.1.1.6
Processing
—
Secondary accessions
A8MPV9, Q6IB07, Q6ICE6, Q9BWC7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative initiation

Biological process

Catecholamine metabolismLipid metabolismNeurotransmitter degradation

Cellular component

Cell membraneCytoplasmMembrane

Ligand

MagnesiumMetal-bindingS-adenosyl-L-methionine

Molecular function

MethyltransferaseTransferase

PTM

Phosphoprotein

Disease

Schizophrenia

Domain

Signal-anchorTransmembraneTransmembrane helix

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