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Mouse PCX (Podocalyxin) ELISA Kit

CAT: 0855-ELK3253-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3253-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PCX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PCX in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Gp200; PCLP; PC; PODXL; Podocalyxin Like Protein 1; GCTM-2 antigen
UniProt
Q9R0M4
Reactivity
Mouse
Field of Research
Tumor immunity; Hematology; Kidney biomarker
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PCX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PCX in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
1.57-100 ng/mL
Sensitivity
0.57 ng/mL

UniProtKB · Q9R0M4

Podocalyxin

PODXL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9R0M4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Podxl
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q9ESZ1
Protein keywords

Biological process

Cell adhesion

Cellular component

Cell membraneCell projectionMembrane

PTM

GlycoproteinPhosphoprotein

Technical term

Reference proteome

Domain

SignalTransmembraneTransmembrane helix

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