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Mouse CBG (Corticosteroid Binding Globulin) ELISA Kit

CAT: 0855-ELK3338-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3338-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CBG in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor, Clade A, Member 6 (Alpha-1 Antiproteinase; Antitrypsin)
UniProt
Q06770
Reactivity
Mouse
Field of Research
Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CBG in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
50 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.79-50 ng/mL
Sensitivity
0.32 ng/mL

UniProtKB · Q06770

Corticosteroid-binding globulin

CBG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q06770
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Serpina6
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
CBG
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Glycoprotein

Ligand

Lipid-bindingSteroid-binding

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

Biological process

Transport

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