Human TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit

Human TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNNT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNNT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNNT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNNT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: ANM; STNT; TNT; TNTS; Slow Skeletal Muscle Troponin T
• UniProt: P13805
• Field of Research: Cardiovascular biology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 1000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 15.63-1000 pg/mL
• Sensitivity: 6.3 pg/mL