Human GLO1 (Glyoxalase I) ELISA Kit

Human GLO1 (Glyoxalase I) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GLO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GLO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLO1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: GLY-I; GLOD1; Glyoxalase Domain Containing 1; Lactoylglutathione lyase; Aldoketomutase; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
• UniProt: Q04760
• Field of Research: Enzyme & Kinase.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 100 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.57 ng/mL