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Human OSTN (Osteocrin) ELISA Kit

CAT: 0855-ELK3820-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3820-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OSTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Musclin
UniProt
P61366
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OSTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6.5 pg/mL

UniProtKB · P61366

Osteocrin

OSTN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P61366
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
OSTN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A1A4U3
Protein keywords

PTM

AmidationCleavage on pair of basic residues

Molecular function

Developmental proteinHormone

Biological process

Differentiation

Technical term

Proteomics identificationReference proteome

Cellular component

Secreted

Domain

Signal

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