Anti-Cyclin D1(DCS-6), CF640R conjugate

• Description:

  Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40% of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_

• Synonyms:

  B cell CLL/lymphoma 1; B cell leukemia 1; B-cell lymphoma 1 protein; BCL-1 oncogene; CCND1 protein; CCND1/FSTL3 fusion gene; CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene; CCND1/PTH fusion gene; G1/S-specific cyclin-D1; Parathyroid adenomatosis 1; PRAD1 oncogene

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CCND1

• Gene ID:

  595

• NCBI Gene ID:

  523852; 667996

• UniProt:

  P24385

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human, Monkey, Mouse, Rat

• Immunogen:

  Human full length recombinant cyclin D1 protein

• Target Antigen:

  Cyclin D1

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  DCS-6

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow, intracellular (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IHC, FFPE, WB

• Field of Research:

  Cancer, Cell cycle

• Positive Control:

  T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  36 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years