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Human THRSP (Thyroid Hormone Responsive) ELISA Kit

CAT: 0855-ELK3878-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3878-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THRSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THRSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THRSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THRSP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
S14; SPOT14; LPGP1; Lipogenic Protein 1; Thyroid hormone-inducible hepatic protein; Spot 14 protein
UniProt
Q92748
Reactivity
Human
Field of Research
Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THRSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THRSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THRSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THRSP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.058 ng/mL

UniProtKB · Q92748

Thyroid hormone-inducible hepatic protein

THRSP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q92748
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
THRSP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R4W7
Protein keywords

Cellular component

CytoplasmNucleus

Biological process

Lipid biosynthesisLipid metabolismTranscriptionTranscription regulation

Technical term

Proteomics identificationReference proteome

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