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Human PSCA (Prostate Stem Cell Antigen) ELISA Kit

CAT: 0855-ELK3884-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3884-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSCA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSCA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSCA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSCA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PSCA
UniProt
O43653
Reactivity
Human
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSCA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSCA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSCA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSCA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.066 ng/mL

UniProtKB · O43653

Prostate stem cell antigen

PSCA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43653
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PSCA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q6UW92
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Domain

Signal

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