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Human ITLN2 (Intelectin 2) ELISA Kit

CAT: 0855-ELK3892-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3892-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITLN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITLN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITLN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITLN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
HL-2; Endothelial lectin HL-2
UniProt
Q8WWU7
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITLN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITLN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITLN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITLN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
3.13-200 ng/mL
Sensitivity
1.04 ng/mL

UniProtKB · Q8WWU7

Intelectin-2

ITLN2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8WWU7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITLN2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q17RR2, Q5VYI0
Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

CalciumLectinMetal-binding

PTM

Disulfide bond

Technical term

Proteomics identificationReference proteome

Cellular component

Secreted

Domain

Signal

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