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Mouse ITGa1 (Integrin Alpha 1) ELISA Kit

CAT: 0855-ELK3916-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3916-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGa1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD49-A; CD49a; ITG-A1; VLA1; CD49 antigen-like family member A; Laminin and collagen receptor
UniProt
Q3V3R4
Reactivity
Mouse
Field of Research
CD & Adhesion molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGa1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
30 pg/mL

UniProtKB · Q3V3R4

Integrin alpha-1

ITA1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q3V3R4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Itga1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
F8VQN5
Protein keywords

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesion

PTM

Disulfide bondGlycoprotein

Molecular function

IntegrinReceptor

Cellular component

Membrane

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

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