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Human HEMGN (Hemogen) ELISA Kit

CAT: 0855-ELK4179-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4179-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HEMGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HEMGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HEMGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HEMGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
EDAG; EDAG-1; NDR; Erythroid differentiation-associated gene protein; Hemopoietic gene protein; Negative differentiation regulator protein
UniProt
Q9BXL5
Reactivity
Human
Field of Research
Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HEMGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HEMGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HEMGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HEMGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.052 ng/mL

UniProtKB · Q9BXL5

Hemogen

HEMGN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BXL5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HEMGN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q6XAR3, Q86XY5, Q9NPC0
Protein keywords

Molecular function

Developmental protein

Biological process

Differentiation

Cellular component

Nucleus

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

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