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Human CRNN (Cornulin) ELISA Kit

CAT: 0855-ELK4187-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4187-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRNN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRNN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRNN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRNN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SEP53; C1orf10; DRC1; PDRC1; 53 kDa putative calcium-binding protein; Squamous epithelial heat shock protein 53; Tumor-related protein; 58 kDa heat shock protein
UniProt
Q9UBG3
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRNN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRNN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRNN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRNN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.117 ng/mL

UniProtKB · Q9UBG3

Cornulin

CRNN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UBG3
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CRNN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2RE60, Q8N613
Protein keywords

Ligand

CalciumMetal-binding

Cellular component

Cytoplasm

Disease

Disease variant

Technical term

Proteomics identificationReference proteome

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