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Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit

CAT: 0855-ELK4298-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4298-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TERF2IP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TERF2IP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TERF2IP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TERF2IP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
RAP1; DRIP5; Dopamine receptor-interacting protein 5; Repressor/activator protein 1 homolog
UniProt
Q9NYB0
Reactivity
Human
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TERF2IP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TERF2IP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TERF2IP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TERF2IP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
1.57-100 ng/mL
Sensitivity
0.67 ng/mL

UniProtKB · Q9NYB0

Telomeric repeat-binding factor 2-interacting protein 1

TE2IP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9NYB0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TERF2IP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1
EC number
—
Processing
—
Secondary accessions
B4DQN4, Q4W4Y2, Q8WYZ3, Q9NWR2
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

Activator

Cellular component

ChromosomeCytoplasmNucleusTelomere

Biological process

TranscriptionTranscription regulation

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