Mouse IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
CAT:
855-ELK4465-01
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
- Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 7732-18-5
- Gene Aliases: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
- Field of Research: Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity.
- Assay Principle: Sandwich ELISA
- Assay Performance Time: 3.5h
- Standard: 1000 pg/mL
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
- Detection Range: 15.63-1000 pg/mL
- Sensitivity: 5.8 pg/mL