Human NTSR1 (Neurotensin Receptor 1, High Affinity) ELISA Kit

Human NTSR1 (Neurotensin Receptor 1, High Affinity) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NTSR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTSR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NTSR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTSR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: NTR; NTRH; NTRR; High-affinity levocabastine-insensitive neurotensin receptor
• UniProt: P30989
• Field of Research: Neuro science.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.061 ng/mL