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Human CS (Citrate Synthase) ELISA Kit

CAT: 0855-ELK4619-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4619-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CS in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Citrate (Si) -synthase; mitochondrial
UniProt
O75390
Reactivity
Human
Field of Research
Enzyme & Kinase; Metabolic pathway; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CS in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
400 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
6.25-400 ng/mL
Sensitivity
2.49 ng/mL

UniProtKB · O75390

Citrate synthase, mitochondrial

CISY_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75390
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.3.1
Processing
Precursor
Secondary accessions
Q71UT9, Q7KZH0, Q96FZ8, Q9BWN8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationMethylationUbl conjugation

Cellular component

Mitochondrion

Molecular function

Transferase

Domain

Transit peptide

Biological process

Tricarboxylic acid cycle

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