Human ABCC13 (ATP Binding Cassette Transporter C13) ELISA Kit

Human ABCC13 (ATP Binding Cassette Transporter C13) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABCC13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABCC13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABCC13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABCC13 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: ABC-C13; PRED6; C21orf73; Putative ATP-binding cassette sub-family C member 13
• Field of Research: Signal transduction.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL