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Human ITGa9 (Integrin Alpha 9) ELISA Kit

CAT: 0855-ELK5040-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5040-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
ITG-A9; Alpha-RLC; ITGA4L; RLC
UniProt
Q13797
Reactivity
Human
Field of Research
CD & Adhesion molecule; Tumor immunity; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.061 ng/mL

UniProtKB · Q13797

Integrin alpha-9

ITA9_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13797
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGA9
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q14638
Protein keywords

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoprotein

Molecular function

IntegrinReceptor

Cellular component

Membrane

Domain

RepeatSignalTransmembraneTransmembrane helix

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