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Human ECE2 (Endothelin Converting Enzyme 2) ELISA Kit

CAT: 0855-ELK5083-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5083-01Size:48 Tests
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ECE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Methyltransferase-like region
UniProt
P0DPD6
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ECE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
10000 pg/mL
Sample Type
Cell lysates and other biological fluids
Detection Range
156.25-10000 pg/mL
Sensitivity
45.4 pg/mL

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