Mouse TGFb2 (Transforming Growth Factor Beta 2) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

• Gene Aliases:

  TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

• UniProt:

  P27090

• Reactivity:

  Mouse

• Field of Research:

  Cytokine, Infection immunity

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  2000 pg/mL

• Sample Type:

  Serum, plasma, cell culture supernates and other biological fluids

• Detection Range:

  31.25 - 2000 pg/mL

• Sensitivity:

  8.95 pg/mL

• Species:

  Mouse

• CAS Number:

  7732-18-5