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Mouse ITGb1 (Integrin Beta 1) ELISA Kit

CAT: 0855-ELK5301-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5301-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD29; MSK12; ITG-B1; FNRB; GPIIA; MDF2; MSK12; VLAB; Fibronectin Receptor Beta Polypeptide; Antigen CD29 Includes MDF2; Glycoprotein IIa; VLA-4 subunit beta
UniProt
P09055
Reactivity
Mouse
Field of Research
CD & Adhesion molecule; Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.111 ng/mL

UniProtKB · P09055

Integrin beta-1

ITB1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P09055
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Itgb1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
F6R105, Q3TIW5, Q3TWH6, Q60993, Q61126, Q8BTU0, Q8BVU1
Protein keywords

Technical term

3D-structureReference proteome

PTM

AcetylationDisulfide bondGlycoproteinIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesionMyogenesis

Cellular component

Cell junctionCell membraneCell projectionEndosomeMembrane

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

IntegrinReceptor

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