Human FOXP1 (Forkhead Box Protein P1) ELISA Kit

Human FOXP1 (Forkhead Box Protein P1) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FOXP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FOXP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FOXP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FOXP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: FOX-P1; 12CC4; QRF1; HFKH1B; Fork Head-Related Protein Like B; Glutamine-Rich Factor 1; PAX5/FOXP1 Fusion Protein
• UniProt: Q9H334
• Field of Research: Apoptosis; Tumor immunity; Immune molecule; Neuro science; Developmental science; Pulmonology; Autoimmunity.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluid
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL