Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin
• Field of Research: Infection immunity; Immune molecule; Kidney biomarker.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 200 µg/mL
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 3.13-200 µg/mL
• Sensitivity: 0.97 µg/mL