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Rat Plg (Plasminogen) ELISA Kit

CAT: 0855-ELK5683-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5683-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Plg in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PL; Plasmin; Activation peptide; Angiostatin
UniProt
Q01177
Reactivity
Rat
Field of Research
Signal transduction; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Plg in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 ng/mL
Sensitivity
6.8 ng/mL

UniProtKB · Q01177

Plasminogen

PLMN_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q01177
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Plg
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5BKB6, Q9R0W3
Protein keywords

Biological process

Blood coagulationFibrinolysisHemostasisTissue remodeling

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Domain

KringleRepeatSignal

Technical term

Reference proteome

Cellular component

Secreted

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