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Rat TPS (Tryptase) ELISA Kit

CAT: 0855-ELK5784-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5784-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPS in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
UniProt
P27435
Reactivity
Rat
Field of Research
Enzyme & Kinase; Infection immunity; Rheumatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPS in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.115 ng/mL

UniProtKB · P27435

Tryptase

TRYB1_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P27435
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tpsab1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.4.21.59
Processing
Precursor
Secondary accessions
P27436
Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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