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Cattle IL6 (Interleukin 6) ELISA Kit

CAT: 0855-ELK5925-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5925-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IL-6; MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
UniProt
P26892
Reactivity
Cattle
Field of Research
Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
3.1 pg/mL

UniProtKB · P26892

Interleukin-6

IL6_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P26892
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL6
Protein existence
2: Evidence at transcript level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
IL-6
EC number
—
Processing
Precursor
Secondary accessions
A9QWQ9, Q08DT2
Protein keywords

Biological process

Acute phase

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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