Cattle MMP1 (Matrix Metalloproteinase 1) ELISA Kit

Cattle MMP1 (Matrix Metalloproteinase 1) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
• UniProt: P28053
• Field of Research: Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 100 ng/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.6 ng/mL