Rat OCLN (Occludin) ELISA Kit

Rat OCLN (Occludin) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OCLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OCLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OCLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OCLN in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Tight Junction Protein Occludin TM4 Minus
• UniProt: Q6P6T5
• Field of Research: Signal transduction.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.32-20 ng/mL
• Sensitivity: 0.122 ng/mL