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Mouse RL (Reelin) ELISA Kit

CAT: 0855-ELK6390-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6390-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RL in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
RELN
UniProt
Q60841
Reactivity
Mouse
Field of Research
Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6.1 pg/mL

UniProtKB · Q60841

Reelin

RELN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q60841
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Reln
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
E9PZ78, Q9CUA6
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-bindingZinc

Biological process

Cell adhesion

Molecular function

Developmental proteinHydrolaseProteaseSerine protease

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignal

Cellular component

Extracellular matrixSecreted