Rat APOF (Apolipoprotein F) ELISA Kit

Rat APOF (Apolipoprotein F) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOF in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Apo-F; LTIP; Lipid transfer inhibitor protein
• UniProt: Q5M889
• Field of Research: Signal transduction; Metabolic pathway; Endocrinology; Cardiovascular biology; Nutrition metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 100 ng/mL
• Sample Type: Serum, plasma and other biological fluids.
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.52 ng/mL